Fresh and frozen Lipogems-derived micro-fractured human adipose tissue generates mesenchymal stem cells with higher differentiation potential and in vivo repair efficacy - Lipogems

Abstract

The differentiation potential and the ease of their isolation have made the multipotent mesenchymal stem cells very important for the development of a vast range of clinical applications in regenerative medicine. Here we report our findings on human adipose tissue-derived stem cells (hADSCs) obtained through the Lipogems device from micro-fractured lipoaspirate. The use of such a device allows the successful establishment of hADSCs colonies even without liberase H1 treatment. Cells maintained in mesenchymal classic medium are large and flat with short outgrowing processes that occasionally grow for several mm. These cells can also be obtained from lipogems preparation after preservation at 4 C for up to 72 hours and, even, cryopreservation at-80 C for over 1 month. Differently it is almost impossible to obtain hADSCs from cryopreserved lipoaspirate. The cell cycle analysis showed that 75% of cells are in G0/G1phase and 21% in S+ G2/M, and only a marginal 0.2% apoptosis. No Chromosomal abnormalities. These hADSCs from either fresh or frozen lipogems preparations are bearer of typical mesenchymal markers at values above 90%, and express embryonic markers such as NANOG and OCT4 and neural markers such as nestin, neurod1, pax 6 and musashi. The superficial epitopes are maintained even when hADSCs were grown in culture after storage at-80 C. Their driven osteogenic and adipogenic differentiation in vitro yields hADSCs with finer intracellular micro-organelles and fat deposits are more numerous and smaller in size. We shall also present how these lipogems-derived hADSCs can transdifferentiate in vivo in …