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Localization of chemotherapy at the tumor site can improve therapeutic efficacy and reduce systemic toxicity. In previous studies we have shown that mesenchymal stromal cells (MSCs) isolated from bone marrow or adipose tissue can be loaded with the anti-cancer drug Paclitaxel (PTX) and kill cancer cells when localized nearby. We here investigated the capacity of human micro-fragmented adipose tissue (MFAT), used as a natural scaffold of MSCs, to deliver PTX with the idea to improve local drug concentration and to prolong the therapeutic activity. Surprisingly, we found that both fresh but also devitalized MFAT (DMFAT) (by freezing/thawing procedure) were able to deliver and release significant amount of PTX, killing several human cancer cell lines in vitro with a long lasting activity. In an orthotopic mice model of Neuroblastoma (NB) transplant, DMFAT loaded with PTX prevents or delays NB relapse when …
Autori
Giulio Alessandri, Valentina Coccè, Fabio Pastorino, Rita Paroni, Michele Dei Cas, Francesco Restelli, Bianca Pollo, Laura Gatti, Carlo Tremolada, Angiola Berenzi, Eugenio Parati, Anna Teresa Brini, Gianpietro Bondiolotti, Mirco Ponzoni, Augusto Pessina
